FastQCFastQC Report
Mon 27 Feb 2023
SRR3127717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895819
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369771.276909917367073No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG33950.11723799035782277No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC30820.10642930376518699Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG29540.10200913800206435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108150.086.72031
TAGGGCA24150.074.534734
ATAGGGC21350.073.963673
TAGGGTA10250.072.9038244
AGGGCAT32500.072.5934455
CGTAGGG7950.071.5323942
ATAGCGG6200.070.5526661
GAATAGG20900.070.215091
CGAAGGG28500.069.920662
AGAGGGC48250.069.15733
AAGAGGG109300.068.7133562
AAGGGCA55700.068.5981454
TAGAGGG56250.068.2627942
AAGGGAT49450.068.2393954
AGGGTAT19350.067.76425
ACGGGTA3350.067.340064
AGTAGGG50500.067.1009142
GTAGGGT10200.066.8105243
AGGGTAG15950.066.5924155
AATAGGG52450.066.308742
Produced by FastQC (version 0.11.5)