FastQCFastQC Report
Mon 27 Feb 2023
SRR3127716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3515714
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501611.4267656584124875No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG37310.10612353564595982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153650.086.3038941
CGTAGGG10550.074.391632
TAGGGCA28100.071.916474
TAGGGTA13300.071.02494
ATAGGGC26950.069.753743
AGGGATG52750.069.403495
TAGAGGG67850.068.7103042
AGTAGGG57850.068.483242
AGGGCAT42700.068.3486565
AAGAGGG131950.068.134282
AAGGGCA71500.068.095744
ACGGGTA5800.068.064014
AGAGGGC57200.068.030023
AGGGTAC16900.067.853055
AAGGGTA28400.067.516214
AAGGGAT63150.067.425024
TAAGGGA35350.067.270913
ATAAGGG56250.066.420892
GGTAAGG21150.066.056261
TAGGGCG6450.065.576634
Produced by FastQC (version 0.11.5)