FastQCFastQC Report
Mon 27 Feb 2023
SRR3127715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720335
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363921.3377764135667114No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG30820.11329486993329867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109650.086.7980651
TAGGGTA9100.076.6221854
TAGGGCA20100.075.94224
ATAGGGC21100.074.352573
AGGGCAT31100.072.380635
CGTAGGG7100.072.327322
TAGACGG2800.070.7088241
AGGGTAT16450.069.9918445
AGAGGGC45300.069.680433
ACGGGTA3350.068.9105154
TAGAGGG50550.068.501552
AAGAGGG98900.068.5009842
GCATAGG7750.068.123771
AAGGGTA22500.067.632314
ATAGCGG6700.067.5427551
GTACGGG6850.067.401532
TAGCGGG13950.067.206782
GGTAAGG16100.067.048951
CGAAGGG28900.066.837512
AAGGGAT43800.066.7961964
Produced by FastQC (version 0.11.5)