FastQCFastQC Report
Mon 27 Feb 2023
SRR3127714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050336
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.8218322517746701No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG35200.3351308533650184No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG34390.32741903543247114No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC33230.316374950492033Illumina Single End Adapter 2 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33040.3145660055448923No Hit
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT12180.11596289187460014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGG4000.083.420942
CGTTTTT34100.083.1945341
AGGGATG25900.079.478755
AAGGGTA9050.079.45474
AGGGCGA4200.079.4485245
GGAGCTA34000.078.0917059
AGTACGG1100.076.9859851
GGATGGC19550.076.6795047
AGGGCAA14000.076.539145
CGAAGGG9000.076.240742
CGGGTAT1800.075.7185445
CGTAGGG2550.075.564952
AGGGCAT9350.074.894755
TAAGCGG2900.074.626261
AAGGGCA16850.074.471154
ATAGCGG2150.074.3998951
AGGGTAT8150.074.3890155
TAGGGCA7950.073.8957754
AGGGTAC4350.073.467695
GAGGGTA5450.073.2991944
Produced by FastQC (version 0.11.5)