FastQCFastQC Report
Mon 27 Feb 2023
SRR3127713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259439
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120450.9563781969591222No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39960.3172841241219305No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG37310.2962430097845152No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC33620.2669442505750576Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG33320.2645622376311993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTC409.094947E-1294.0048455
AGGGATG30050.082.117385
CGTTTTT48350.081.6491241
TAGCGGG6100.080.138462
GGAGCTA39200.079.8498159
AGGGCAT13500.079.033715
GGTAAGG5900.078.95311
AGGGTAC4300.078.701735
AGGGCGA6000.078.3373645
CGAAGGG11700.077.536992
TAGGGCA9250.077.239484
AAGGGCA21750.077.151874
ATAGCGG2750.076.995591
AAGGGTA12400.076.950574
ATAGGGA14300.076.58743
AAGCGGG15600.076.532622
AGAGGGC19150.074.863223
GGATGGC25200.074.607027
TAAGCGG3050.074.050411
GGGCGAT4650.073.788756
Produced by FastQC (version 0.11.5)