FastQCFastQC Report
Mon 27 Feb 2023
SRR3127712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978231
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85850.8776045739707697No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG31290.3198630998199811No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG30030.3069827065386397No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30000.3066760305081315No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC28170.2879687926471355Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT11020.11265232853998697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT34550.082.9255751
TAGGGCA7050.082.840574
ACGGGTA1500.081.638044
AGGGATG22700.081.3619845
AGGGTAC4250.080.721435
TAGCGGG4400.078.1413042
CGAAGGG8450.078.033622
TAAGGGT2750.077.070883
CGCTACG5200.076.988533
TACGAGG1600.076.574891
CGAGGGA5150.075.906913
GAGGGTA5550.075.5279164
GGAGCTA22850.075.274829
CGGGTAT2000.075.192565
AAGGGCA17250.075.085154
GGTAAGG5100.074.842421
TAGGGCG1200.074.573214
GCGGGAT4550.074.530084
AAGGGTA8350.074.455694
AAGCGGG11900.074.012512
Produced by FastQC (version 0.11.5)