FastQCFastQC Report
Mon 27 Feb 2023
SRR3127711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465557
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236330.9585258016748345No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG29900.12127077167552809No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27980.11348348466492561No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG25970.10533116857570114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69700.083.535031
CGTAGGG6200.076.560022
TAGGGCA16750.069.5825654
ATAGGGA21300.069.2809143
AGTAAGG16600.069.129351
GAATAGG15650.069.118491
TAGCGGG11200.068.817392
GGTAAGG13300.068.247451
CGAAGGG24250.068.024872
GGATAGG17450.067.918151
GCGTAGG3550.067.5651251
AAGAGGG102500.067.309222
ATAGAGG18600.067.258851
TATAGGG22700.066.665692
AAGGGCA51150.066.612534
ATAAGGG37800.066.5172652
GAGGGAT31700.066.269214
AGGGCAT27450.066.088535
TAAGGGA22900.066.082123
AGGGATG32600.066.0281455
Produced by FastQC (version 0.11.5)