FastQCFastQC Report
Mon 27 Feb 2023
SRR3127710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971678
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312441.0513925129169446No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34640.11656713816234465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95900.084.809811
ATAGGGA26950.074.6379853
TAGGGCA22000.072.846154
ATAGGGC21550.071.3140953
CGTAGGG9100.070.2380452
GAATAGG19400.069.5904851
AGTAGGG50350.069.352842
AGATAGG19650.069.183891
AGTAAGG20700.069.0832751
AGGGTAC15600.068.9899445
GGATAGG20250.068.527781
ATAGCGG6400.068.355371
AGGGCAT34950.068.176665
TAGCGGG15200.067.713532
TAAGGGA33200.067.5235443
GATAGGG48650.067.332522
AAGAGGG121100.067.10052
AAGGGCA61700.066.573474
GTAGGGA27150.065.952323
AGGGATG38850.065.808625
Produced by FastQC (version 0.11.5)