FastQCFastQC Report
Mon 27 Feb 2023
SRR3127708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410455
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239690.9943765803551611No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG39640.16445028013383364No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC36560.15167260952807665No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33450.13877048109174409No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG30850.12798413577519596No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30780.12769373417051968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67400.084.58021
ATAGGGC14650.075.706093
CGTAGGG6650.074.910152
GAATAGG13200.073.047591
TAGGGCA13000.073.022294
AGGGCAT22400.069.862425
AGTAAGG15000.069.299011
GGTAAGG13900.068.692161
ACGGGTA2200.068.3557054
TAGAGGG43250.068.3472442
AGTAGGG37150.068.1846162
AAGAGGG87500.067.2975852
TAGGGCG3850.067.135064
AGGGAAT22600.066.956835
AGATAGG15400.066.888181
TAGTAGG10400.066.48291
AAGGGCA38150.066.395964
AGGGATG24350.066.3906565
AGAGGGC39800.066.006273
GGATAGG12000.065.849741
Produced by FastQC (version 0.11.5)