FastQCFastQC Report
Mon 27 Feb 2023
SRR3127707.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127707.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823369
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309211.0951809699688564No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41140.14571244495494567No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37520.13289088319663495No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG33910.12010474011721459No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC32440.11489819432033148No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG29320.10384756650653883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88350.084.965961
ATAGGGC18000.075.980233
CGTAGGG6750.074.500662
TAGGGCA15650.073.875574
AGGGCAT27850.070.539315
AGGGAAT23950.069.6631245
AGTAGGG42650.069.312532
GAATAGG16650.069.210261
TAGTAGG12350.068.9334951
GGATAGG17550.068.609061
ATGCGGG14300.068.032182
AAGAGGG102700.067.8200152
TAGACGG3850.067.1924741
TAGAGGG53600.066.9021152
GATAGGG41250.066.651822
ATAAGGG41850.066.4823462
AGTAAGG15850.066.175041
ATAGGGA20400.065.8894653
ATAGAGG20050.064.511471
TATAGGG22600.064.258442
Produced by FastQC (version 0.11.5)