FastQCFastQC Report
Mon 27 Feb 2023
SRR3127706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177858
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233811.0735777998381897No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG34870.16011144895580887No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC31670.1454181126593194No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29500.13545419398326247No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29000.13315836018693597No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG28640.13150535985358092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68150.084.2437061
ATAGGGC13900.076.942643
TAGGGCA11700.074.900234
ATAGCGG4400.073.918381
ATAACGG1150.073.778651
GAATAGG11900.073.6753161
ACGGGTA2600.072.484094
TAGTAGG8800.071.240181
CGTAGGG5050.070.9052352
CGGGTAT1200.070.491895
AGTAAGG12000.070.311741
AGTAGGG34650.070.162092
TAGAGGG38450.069.1096652
TAGGGCG3300.068.530414
AAGAGGG76900.067.577982
AGGGCAT19900.066.8315055
AGAGGGC31550.066.453323
AGGGAAT19900.065.178435
AGGGATG23750.065.099885
ATAGAGG15000.064.733931
Produced by FastQC (version 0.11.5)