FastQCFastQC Report
Mon 27 Feb 2023
SRR3127705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569894
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308081.1988043086602016No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG29930.11646394754024875TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG28480.11082169147832556TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC28180.10965432815516905TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89500.086.382371
TAGGGCA19900.073.922314
AGTAGGG41700.071.681212
AGGGCAT30200.071.275925
AGGGATG37850.070.9013445
TAGAGGG46450.070.421912
AGGGTAC13200.070.141755
ATAGGGC20950.069.5443653
AAGAGGG99550.069.258512
AGAGGGC42650.068.9826053
CGAAGGG26450.068.765342
TAGGGTA8600.068.311814
CGTAGGG6750.068.23492
GAGGGAT37500.068.053884
TAAGGGA22350.067.921823
AAGGGCA51000.067.641024
GTAAGGG41500.067.609932
AGTAAGG18300.067.3281251
TAGGGCG5300.066.5073554
AAGGGAT47900.066.42594
Produced by FastQC (version 0.11.5)