FastQCFastQC Report
Mon 27 Feb 2023
SRR3127703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424092
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297961.2291612694567697No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG26760.11039184981428098TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG25660.1058540682449346TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC25600.10560655288660661TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.086.824951
TAGGGCA16100.072.871614
ACGGGTA2700.071.549244
AGGGATG36000.071.405325
TAAGGGA22500.070.781393
AGGGCAT29300.070.7321245
TAGAGGG44350.070.650122
AAGAGGG98250.070.640722
TATAGGG17000.070.122352
AGTAGGG37500.069.985622
AAGGGCA50900.069.797274
CGAAGGG23900.068.606652
GAATAGG16850.068.544981
AGAGGGC41750.068.504183
AGAGGGA57550.068.28193
TAAGAGG19850.068.1601941
ATAGGGC18150.068.015763
AGGGTAC13900.067.955875
GAGGGAT33700.067.530844
CGTAGGG6700.067.5120542
Produced by FastQC (version 0.11.5)