FastQCFastQC Report
Mon 27 Feb 2023
SRR3127702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247614
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237731.0576994092401986No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40200.17885633387227523No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC29030.12915918836597387No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24760.1101612643452123No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG23400.10411040329878707No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG22520.10019514026874722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72100.084.363221
ATAACGG2650.074.557831
ATAGGGC14800.073.989213
CGTAGGG7200.073.7598652
ATAAGGG30300.071.8145752
AGTAGGG37950.069.969842
GAATAGG13350.069.770811
AGGGATG34150.069.3623355
TAAGGGA17200.068.583483
AAGAGGG85050.068.023342
ATAGGGA17600.067.825853
GTAGGGT7450.067.499693
TAGGGTA7550.067.228134
ATAGCGG4400.066.287081
TAGGGAT12850.066.198734
TAGTAGG11700.065.9398351
AGATAGG12800.065.785921
AGAGGGC35150.065.783043
CGAAGGG24000.065.600592
TAGGGCA11900.065.5594564
Produced by FastQC (version 0.11.5)