FastQCFastQC Report
Mon 27 Feb 2023
SRR3127701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741698
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313641.1439626100321771No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52570.1917424895083266No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30540.11139082422644653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94900.083.778871
CGTAGGG8300.076.439522
ATAGCGG6050.073.8724061
TAGGGCA15050.072.758164
ATAGGGA21400.071.592273
ATAGGGC18000.071.2775043
ACGGGTA3500.069.822864
GAATAGG17750.069.176111
GGTAAGG14350.067.5350651
AGTAGGG45300.067.433782
AGGGATG39950.067.2886055
GTAGGGT9450.067.137363
TAGGGTA9450.067.137364
AAGGGCA51250.067.124264
TAGAGGG51750.067.111412
CGAAGGG31950.066.4859542
ATAAGGG38950.066.241052
AGAGGGC41600.065.9753653
TAGGGCG4850.065.891524
AGGGCAT28150.065.610965
Produced by FastQC (version 0.11.5)