FastQCFastQC Report
Mon 27 Feb 2023
SRR3127700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050872
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234101.14146567898923No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36070.175876407693898No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC27580.13447938242854746No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22290.10868547622669772No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG21940.10697888507912733No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG21700.10580865114936475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71250.085.000251
CGTAGGG6300.077.7684862
TAGGGTA6200.072.944144
GAATAGG12450.072.304611
ATAGGGA16050.071.912093
ATAGCGG5600.071.537351
ATAACGG1450.071.508341
TAAGGGA16400.071.239153
ATAAGGG29100.069.9361342
AGTAGGG32500.069.287582
AGGGAAT19750.068.767235
ATAGGGC12900.068.656083
TAGAGGG37450.068.3062
AGGGCAT20000.068.142625
GTAGGGT5950.068.091423
TAGGGCA12050.067.2438054
AGGGATG29800.067.180635
AAGAGGG77000.066.81022
AGTAAGG13750.066.496841
ACGGGTA2500.065.953674
Produced by FastQC (version 0.11.5)