FastQCFastQC Report
Mon 27 Feb 2023
SRR3127699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608213
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230080.8821365432961189No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG32380.12414630246839503No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG31290.11996719593070045No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC29330.11245247224824047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76100.085.164481
ATAGGGC21350.074.1852953
AGGGTAC11850.074.1695565
TAGGGCA19100.074.067434
AGGGATG46550.073.807445
ATAGCGG6500.073.804151
CGTAGGG9250.073.1655352
AGTAGGG44650.072.945352
TAGCGGG14500.071.632492
TAGGGTA10450.071.061594
AAGGGAT54100.070.803464
TAGGGAT24400.070.6920244
AGGGCAT32150.070.171825
AGAGGGC46200.069.98943
AGGGATC25850.069.8189245
GAGGGAT42750.068.712864
ATAGGGA30650.068.6963043
AAGGGCA54400.068.5122764
AGTAAGG20400.068.473141
TATAGGG22550.067.736492
Produced by FastQC (version 0.11.5)