FastQCFastQC Report
Mon 27 Feb 2023
SRR3127697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442123
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227160.9301742786911223No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG28870.11821681381322727No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG27300.11178798119505037No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC26480.1084302469613529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74950.085.447131
TAGGGCA16600.077.200724
CGTAGGG8250.077.09752
GTAGGGC16100.076.086573
AGGGATG44000.075.511725
ATAGCGG5550.073.871341
AGGGTAC10300.072.5450065
AGTAGGG42350.072.091182
ATAGGGC15300.072.058463
AGGGCAT29500.071.368025
CGAAGGG26200.071.2122042
GTACGGG5000.070.6727142
AAGGGCA50600.069.7415854
AAGGGAT56050.069.348784
TAGCGGG13700.069.125142
AGAGGGC44150.068.511723
GAGGGAT39550.068.2603764
ACGGGAT9800.067.788124
AGGGATC26200.067.2632145
TAGAGGG45050.067.247582
Produced by FastQC (version 0.11.5)