FastQCFastQC Report
Mon 27 Feb 2023
SRR3127694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996730
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT797353.9932790111832843No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT86300.432206657885643No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT79440.39785048554386426No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT54770.2742984780115489No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG42910.2149013637296981TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC39840.19952622537849382No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC32160.16106333855854324No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC26360.13201584590805968TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT220300.090.840741
TAGGGCA11250.074.138174
GAATAGG10100.073.281731
CGTTTCT12500.072.411681
ATAGCGG4000.071.8931051
AGGGCAT20550.070.891615
TAGCGGG10200.070.22062
GGTAAGG11750.069.410581
ATAGGGC13800.068.9752663
AGTAGGG30300.068.583092
TAGAGGG35400.068.419672
AGAGGGC31100.067.4249653
AGGGATG25350.066.922825
ATAGGGA13750.066.827163
AAGGGCA37450.066.687614
ACGGGTA2900.066.620364
AGATAGG12250.066.192651
GTTTTTT302150.066.109212
ATAAGGG26500.065.792592
GTAGGGC10150.064.995473