FastQCFastQC Report
Mon 27 Feb 2023
SRR3127693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593476
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338571.305468028237007No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG28220.10881149468898112No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG26970.10399170842529486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112350.087.37191
CGTAGGG6600.075.483022
AGGGATG51200.074.627625
ATAGGGC19700.073.95763
ATAGGGA31500.072.960143
TAGGGCG6000.072.846834
TAGGGCA18700.071.62794
AAGGGAT60850.071.597544
CGAAGGG26150.071.531732
AAGGGCG12450.070.213814
GAATAGG14550.070.148631
GTAGGGA23600.069.900853
AGTAGGG45800.069.779972
AAGGGCA50200.069.747964
GTACGGG5800.069.6879652
AGGGCAT29900.069.632425
ATAGCGG4000.069.376821
AGAGGGC47300.069.156893
ATGAGGG47800.068.925112
TAGGGAT22750.068.792624
Produced by FastQC (version 0.11.5)