FastQCFastQC Report
Mon 27 Feb 2023
SRR3127692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3106295
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437331.4078830246322387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151550.087.506461
ATAGGGC22550.078.155423
CGTAGGG8850.077.532532
AGGGATG65950.075.537995
TAGGGCA22650.075.3204354
ATAGCGG5700.075.1047741
AAGGGAT78400.073.673314
TAGGGCG6700.073.6527254
TAAGGGA40400.073.5207753
ATAGGGA40550.072.4375153
TAGAGGG60400.070.340572
TAGGGAT33500.070.285754
CGAAGGG31700.070.27382
AGGGATT50550.069.636195
AGAGGGC56000.069.1534043
AGGGCAT35400.069.035815
AAGGGCG16400.068.7767644
GGTAAGG19050.068.651631
GAGGGAT54450.068.618874
AGAGGGA87700.068.272253
Produced by FastQC (version 0.11.5)