FastQCFastQC Report
Mon 27 Feb 2023
SRR3127689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3602581
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359370.9975348229505456No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG44260.12285636325734245Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC43950.12199586907275645TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG39920.11080944467313852Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120300.085.374821
CGTAGGG11400.077.50512
TAGGGCG8800.076.905454
TAGGGCA28950.075.0016254
AGGGATG76850.074.852935
AGGGCAT46950.073.3735665
AAGGGCA77000.072.388784
ATAGGGA48150.072.033973
ATAGGGC28800.071.965343
TAGGGAT43500.071.523044
ATAGCGG7800.071.1752551
AAGGGAT90750.070.898024
CGAAGGG35950.070.856172
AGTAGGG66300.070.03592
AGAGGGC62000.069.208013
TAAGGGA42700.069.010783
CGAGGGA23700.069.0093843
GTAGGGA37550.068.838283
TAAGCGG9300.068.801461
ACGGGTA4800.068.538424
Produced by FastQC (version 0.11.5)