FastQCFastQC Report
Mon 27 Feb 2023
SRR3127688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764688
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260060.9406486373869313No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG37460.1354944934111914Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG37030.13393916420225357Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC34110.12337739376016389TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88300.086.225171
ATAGCGG6300.076.327651
CGTAGGG8600.076.159822
TAGGGCA21250.076.057974
CGAAGGG26300.073.2785952
TAGCGGG14800.073.2277452
AGGGATG62700.073.1522755
TAGGGCG5450.072.626044
CGGGATG11350.071.630145
AAGGGAT68800.070.886164
AAGGGCA52850.070.88134
TAAGCGG8450.070.85481
TAAGGGA31500.070.755493
CGAGGGA14500.069.868283
ATAGGGC24100.069.800863
GGTAAGG17000.069.6060641
AGGGCAT33850.068.999075
ATAGGGA34500.068.9734343
AGAGGGC44800.068.682283
AGGGATT41300.068.0450745
Produced by FastQC (version 0.11.5)