FastQCFastQC Report
Mon 27 Feb 2023
SRR3127687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313929
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91570.6969174133457744No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG40930.31150846050281256No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG38640.29407981709818415No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC33690.25640654860346335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43550.085.323681
GCGTATT401.036824E-982.247278
CGAAGGG12650.080.999532
TAAGCGG2100.080.6331941
ATAGCGG2750.076.968051
TACGAGG1600.076.433551
CGAGGGA8500.076.309163
GTACGGG2350.076.0032652
TAGGGCG1550.075.809714
GGGCGAT5850.075.518866
AACGAGG4950.075.06761
CGTAGGG3950.074.965242
AGGGCAT14300.074.9402855
TAGCGGG6100.074.740912
AAGGGAT33150.074.437594
AGGGATT21300.074.36475
AGGGATG22550.074.202745
AGAGGGA37050.073.579413
AAGGGCG6150.073.3694
AAGGGCA19500.072.31084
Produced by FastQC (version 0.11.5)