FastQCFastQC Report
Mon 27 Feb 2023
SRR3127686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584431
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125000.788926750360224No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG40240.2539712994759633No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG39650.25024756521426306No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC33580.21193728221677055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53500.085.989481
AGGGCGT2750.080.315685
CGAAGGG12150.080.062512
GCGTAGG1900.079.223971
TAGGGCG2750.078.6068344
TAGCGGG8650.078.2314762
AATGCGG2300.077.7169951
CGTAGGG3750.077.695452
ATAGCGG4000.075.262771
AGGGATT31650.073.199545
TGTACGG1100.072.6971
AAGGGAT43200.072.4478764
AGGGATC21650.072.06355
TAGGGCA12400.072.005734
CTAACGG1050.071.678831
CGAGGGA9800.071.448873
AGAGGGA47000.071.089743
AAGGGAC18750.070.6777954
GGAGCTA32900.070.561249
GCGGGAT8400.070.489824
Produced by FastQC (version 0.11.5)