FastQCFastQC Report
Mon 27 Feb 2023
SRR3127685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208517
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90130.7457900881824584No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG36340.3006991213197663No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG33110.2739721493367491No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC29020.24012901763069947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37600.085.8504941
CGAAGGG8750.079.138392
AGGGATT23750.077.9623955
CGAGGGA8100.076.184083
CGTAGGG2350.076.171712
AAGGGAT33850.076.1213764
AGGGATG22050.076.0872965
TAGCGGG6000.075.36992
ACGGGTA1150.073.731424
TAGGGCG1800.073.276294
AGGGCGA6250.072.936395
AGTAGGG19850.072.6170962
GGGCGAT5850.072.300156
AGGGATC16800.072.171045
GCGGGAT6600.072.086754
ATAGCGG2550.072.071661
GAGGGAT27450.071.9034354
TAGGGCA9050.071.830434
TGCGGGC2650.071.103683
AAGGGCG5900.071.058494
Produced by FastQC (version 0.11.5)