FastQCFastQC Report
Mon 27 Feb 2023
SRR3127684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891791
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437391.5125228621293862No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG34440.11909574377954701No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC33950.11740129214040709No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG31130.10764955005392851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT130300.087.939051
CGTAGGG7650.076.181332
TAGGGCA18750.075.698364
AAGGGTA30400.075.5994
AGGGTAC26450.075.16285
ATAGGGC18700.073.640083
TAGGGTA11800.072.886964
GAATAGG19750.072.8791351
GTACGAT1750.072.508928
ATAGGGA25750.071.0003743
AGGGTAT21350.070.883685
GGTAAGG18650.070.620061
AGTAAGG20750.069.820271
GAGGGAT32950.069.463124
AGAGGGC41950.069.462113
TAGAGGG54200.069.371162
AGTAGGG51750.069.204272
GAGGGTA26750.069.047754
CGAAGGG29650.068.952952
AGGGTAG15250.068.726365
Produced by FastQC (version 0.11.5)