FastQCFastQC Report
Mon 27 Feb 2023
SRR3127682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736990
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424931.552544949013332No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC31450.11490725212733696No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG31330.11446881428138209No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG27910.10197333567166851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126100.087.840881
CGTAGGG8000.077.732942
AGGGTAC26100.077.604255
AAGGGTA32700.075.204514
AGTAAGG17700.074.297611
ATAGGGA23950.073.3713
TAGGGCA19700.072.6990054
ATAGCGG5050.071.869271
ATAGGGC19600.071.868123
TAGGGTA14250.071.4101644
AGGGTAT19200.070.981555
AGAGGGC43100.070.611663
GAATAGG18450.070.5109561
GCATAGG7900.070.404241
AGTAGGG46950.070.239852
AGGGCAT30750.069.842515
GGTAAGG19050.069.5272751
AGGGCAC26250.069.4626165
AAGGGCA49300.069.376254
CGAAGGG25750.069.339882
Produced by FastQC (version 0.11.5)