FastQCFastQC Report
Mon 27 Feb 2023
SRR3127681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399437
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130520.9326607771553845No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG46950.3354920585921338No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG41130.2939039056420546No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC38330.273895859549233TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT17630.1259792330773018No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16710.11940516078966042No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT14930.10668576005922381No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT14590.10425621160509548No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14410.10296998007055694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1200.086.1610954
CGTTTTT44300.085.683391
CGTAGGG3550.084.726922
GGTACGA600.078.325477
AAGGGCA23100.075.276524
AGGGATG23600.075.2747965
TAACGGG3600.071.800922
GAAGGGC17400.071.575833
CGAAGGG11500.071.517122
AATGCGG2400.070.553421
TGACGGG4350.070.225342
TAGAGGG19600.069.776112
TAGGGCA9050.069.586664
TAAGGGA12650.069.473773
AAGGGTA10650.068.840624
AGGGTAT8750.068.749845
AGAGGGC17900.068.263753
ATAGGGA14900.068.129823
AGTACGG1450.068.120551
AGGGCAT12550.067.780485
Produced by FastQC (version 0.11.5)