FastQCFastQC Report
Mon 27 Feb 2023
SRR3127680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661964
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175551.0562804007788376No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG43590.26228004938735133No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG42990.2586698628851167No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC38280.23032989884257418TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21490.1293048465550397No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17750.10680135069111003No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT16890.10162675003790696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60750.085.022171
AGGGATG31000.078.078155
AAGGGCA27550.075.74354
TAGAGGG29200.075.0044562
CGAAGGG13550.074.920112
CGTAGGG5250.074.302382
ATAGGGA17950.073.3124163
AGGGCAT15050.073.074075
ACGGGTA2000.072.847674
TAGCGGG5950.072.669942
ATAGCGG2400.072.521471
TAGGGCA10700.071.595834
AGTAAGG12300.070.370211
AGGGCAC13050.069.5073555
TAAGGGA16550.069.006863
GAGGGAT27000.068.757064
GGTAAGG9800.068.6413961
TAGGGAG11650.068.58154
AGAGGGC21950.068.303053
AGGGAAT20250.068.234865
Produced by FastQC (version 0.11.5)