FastQCFastQC Report
Mon 27 Feb 2023
SRR3127677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719191
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303821.1173176139520908No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32520.11959439406794153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90750.084.758471
AGTAGGG44950.073.609092
TAGGGCA20000.073.552064
ATAGGGC21400.071.595273
ATAGGGA24400.071.460263
AGGGCAT31750.070.310865
GAATAGG17550.069.696051
TAGAGGG54600.068.776912
CGTAGGG6700.068.744792
AAGGGCA56700.068.632184
ACGGGTA4950.068.360914
AGGGTAC12700.068.0905155
GGTAAGG15400.067.817891
AAGAGGG108550.067.500162
CGAAGGG27800.066.94822
ATAGAGG19650.066.796491
GAGGGAT32200.066.556354
TAGTAGG12800.066.5243451
AGATAGG18550.065.685251
TAGCGGG14250.065.6336752
Produced by FastQC (version 0.11.5)