FastQCFastQC Report
Mon 27 Feb 2023
SRR3127676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080911
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225621.0842366636535632No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24080.11571854827044502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66300.085.002511
TAGGGCA14600.073.556034
AGTAAGG12850.072.9733051
ATAGGGC17050.072.37913
AGTAGGG36350.071.008842
AAGGGTA14600.070.975114
ATAGGGA18250.070.458933
GGTAAGG13600.070.334951
GAATAGG13150.070.233511
ATAGAGG14950.069.341871
ATAAGGG31750.068.390142
CGTAGGG5650.068.359942
AAGGGCA43700.068.011794
TAGAGGG40000.067.944152
GAGGGAT23150.067.142764
AGGGTAC8350.066.975315
GCATAGG5700.066.961361
TATAGGG17450.066.941052
ATAGCGG5000.066.911761
AAGAGGG85600.066.9107742
Produced by FastQC (version 0.11.5)