FastQCFastQC Report
Mon 27 Feb 2023
SRR3127675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128041
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290340.9281847648416374No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC58190.18602697343161423TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG55960.17889791086497908No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG55060.17602071072597833No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31930.10207666715365943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83900.083.246571
TAGGGCA24100.077.0329064
TAGCGGG14700.075.776442
CGGGTAT3450.074.9260565
ACGGGTA3150.074.60294
CGTAGGG8300.074.1816252
ATAGCGG5400.072.291321
AGTAAGG21700.071.52471
ATAGGGA26750.070.631513
AAGGGTA27600.070.3294454
ATGCGGG16600.069.9345862
AAGGGCA57900.069.079194
AGTAGGG49000.068.1028752
ATGTAGG11700.067.9364241
CGAAGGG29900.067.749992
AGGGCAT34100.067.5353245
GTAGGGC18800.067.499753
AAGAGGG120250.067.266522
TAGAGGG59100.067.2004852
AGAGGGC51450.067.0512543
Produced by FastQC (version 0.11.5)