FastQCFastQC Report
Mon 27 Feb 2023
SRR3127674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736077
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393241.0525478998425355No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC56260.15058576148189665TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG52880.14153883873378414No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG51570.1380324870177997No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40450.10826864649738215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118600.084.046111
TAGGGCA29550.075.702124
TAGCGGG17500.075.461742
CGTAGGG11550.074.460792
AAGGGTA31400.070.792954
GAATAGG25150.069.5787051
AGAGGGC62900.069.1861043
TAGAGGG71000.068.971222
AGGGCAT43400.068.7610555
AGGGTAT24150.068.693575
CGAAGGG36150.068.641092
AAGGGCA72750.068.53954
TAGGGCG6650.067.8434754
ATAAGGG56900.067.6441352
GATAGGG57600.067.637972
ATAGGGC28950.067.531013
ATAGGGA34900.067.194493
AGGGATG54850.067.17355
AAGAGGG137950.066.7717742
AGGGTAA21400.066.7603155
Produced by FastQC (version 0.11.5)