FastQCFastQC Report
Mon 27 Feb 2023
SRR3127673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864405
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281800.9837994278043781No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG47570.16607288424646655No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC47430.1655841265463508TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG47400.16547939275346887No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29550.10316278598871317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83300.084.687811
TAGGGCA20200.078.579614
TAGCGGG12650.076.329892
CGTAGGG8050.071.9681852
TAGAGGG55100.071.634752
AAGGGTA24650.070.890554
AGGGTAT18600.070.745275
AAGGGCA52950.070.6294564
ATAGGGC20650.070.480633
CGAAGGG28850.070.365992
AGGGCAT32000.069.905175
AGTAAGG18900.069.065651
GTAGGGC18050.068.629343
GAATAGG17900.067.922051
ATAGGGA26300.067.875773
ATAAGGG41700.067.658462
AAGAGGG107650.067.55612
AGTAGGG48750.067.4391562
ATAGCGG6150.067.429771
AATGCGG6500.067.423881
Produced by FastQC (version 0.11.5)