FastQCFastQC Report
Mon 27 Feb 2023
SRR3127672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389229
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124690.8975482083947283No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG35800.2576968951843073No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG34530.24855513381883046No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC33640.24214870262570104No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24800.1785162849321458No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41850.085.964411
CGTAGGG4150.084.937612
AGGGATG29750.076.145985
ATAGCGG3000.075.244141
AGTAAGG8650.075.026671
AATGCGG2350.074.0434341
AGGGCAT15000.073.944795
TAGGGCA10200.073.2628564
AGGGTAA6400.072.701295
TAGCGGG7000.072.512452
AGAGGGC19800.072.3971563
CGAAGGG12950.072.2221152
AAGGGCA24100.072.155854
GTAGGGA11200.071.757123
CGAGGGA7750.071.559483
AGGGTAC7450.071.286795
AGAGGGT13050.070.588263
GAGGGAT21800.070.282624
AAGGGCG6300.070.125214
GAAGGGC21150.069.998233
Produced by FastQC (version 0.11.5)