FastQCFastQC Report
Mon 27 Feb 2023
SRR3127671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672889
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165500.9893065230269312No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC36540.2184245338453418No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG34680.2073060436167612No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG33160.19821996558050176No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32090.19182384485760862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56000.085.9424741
ATAGCGG3250.079.615861
CGTAGGG4800.079.3066252
AGGGATG38950.077.342155
GAGGGAT29350.074.778114
CGAAGGG14050.074.592352
TAGGGCA13100.074.261674
AGGGCAT18450.073.360425
AAGGGCA28400.072.81154
GCGTAGG2400.072.528851
TAGCGGG8400.072.1732252
AGAGGGA45150.071.613743
AGGGTAC9400.071.49475
AGGGTAA8000.071.082235
AGAGGGC22400.070.914393
GAATAGG10100.070.8015061
AAGAGGG60650.070.514152
GTACGGG4050.069.624472
TAAGGGA18350.069.40633
AGAGGGT16350.069.2731553
Produced by FastQC (version 0.11.5)