FastQCFastQC Report
Mon 27 Feb 2023
SRR3127670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293642
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118560.916482303450259No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG29330.22672424055496035No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG28570.22084935399438177No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC26860.20763085923307992No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22570.17446867062139293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41000.085.190341
ATAGCGG2950.084.685641
CGTAGGG3650.080.04552
TAGCGGG6650.078.657372
AGGGTAC7050.078.657035
AATGCGG1800.078.5605851
AGGGATG27550.077.783575
GAGGGAT21400.076.410644
GGATGGC19800.075.00097
GTACGGG2400.074.612312
CGAGGGA7300.074.235753
GGTAAGG7450.073.393511
CTAACGG900.073.323211
TAGGGCA9000.072.7797244
TAGGGCG2550.072.071334
AAGGGTA10500.071.8073354
AGGGCGA6300.071.6102755
AAGGGCA20600.071.6003654
GTAAGGG21900.070.577752
AAGAGGG45850.069.683262
Produced by FastQC (version 0.11.5)