FastQCFastQC Report
Mon 27 Feb 2023
SRR3127669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065849
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196400.9506987199935717No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG40940.19817518124509584No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC40650.1967714000394027TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG38630.18699333784802277No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25440.12314549611322027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60400.084.567581
TAGGGCA14050.076.2663654
AGTAGGG35550.074.168372
CGTAGGG5400.072.240352
TAGGGTA7150.071.646494
ATAGGGC13350.069.3520663
GAGGGAT24200.068.942574
TATAGGG18200.068.43372
ATAAGGG28350.068.13722
GAATAGG11400.067.66281
AAGAGGG71250.067.481742
AGGGAAT20700.067.4327855
AATAGGG31650.067.4182742
AGTAAGG13400.067.391861
ATAGAGG13900.067.33631
AGACGGG13300.067.1424942
TAAGGGC16950.066.545093
ATAGGGA17650.066.302383
TAGCGGG8800.066.226912
AGATAGG12150.066.195861
Produced by FastQC (version 0.11.5)