FastQCFastQC Report
Mon 27 Feb 2023
SRR3127668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477723
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263951.0652926093836963No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC41340.1668467379121879TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG39740.1603891960481458No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG38570.15566711856006502No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31140.1256799085289195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72900.085.356441
CGTAGGG6600.079.038932
TAGGGTA7850.079.025344
GAATAGG13850.072.332561
TAGGGCA15600.070.4941944
ATAGGGC14200.070.494193
ATAGGGA21450.069.891673
AGTAGGG43400.069.519622
TAGACGG3400.069.166391
AGTAAGG17600.068.411841
ATAAGGG33600.068.396152
TAGAGGG44400.067.953862
TATAGGG23600.067.706282
ATAGAGG17150.067.464451
AGAGGGC36250.066.3779753
AGGGTAT14850.066.142695
TACGAGG8050.066.0216751
GGTAAGG12650.065.809221
ACGGGAT5800.065.6325154
GTAGGGT11050.065.4968643
Produced by FastQC (version 0.11.5)