FastQCFastQC Report
Mon 27 Feb 2023
SRR3127666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676165
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260080.971838432981524No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35460.13250304073179345No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG31490.11766837993920404No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG30400.11359538742939991No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC29900.11172704224141636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83250.086.2103961
AGGGTAC15100.075.3215565
AGGGATG54050.074.5188145
AAGGGTA24200.073.604554
CGAAGGG27200.073.0904162
CGTAGGG7500.072.065192
ATAGGGC19750.071.627583
ACGGGTA4200.071.616254
AGTAGGG44300.070.869882
AGGGCAT28800.070.4972465
TAGAGGG51350.070.2010962
TAGGGCA18050.069.7812044
ATAGGGA28100.068.908333
AAGGGAT56700.068.798014
AAGGGCA44750.068.370514
TAACGGG8800.068.3622362
TAGGGTA13050.068.06634
AGGGTAT19900.068.017435
GAGGGTA20850.067.8486564
AGGGTAA18300.067.543815
Produced by FastQC (version 0.11.5)