FastQCFastQC Report
Mon 27 Feb 2023
SRR3127664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499761
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251621.0065762286874624No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33720.13489289576083474No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG27510.11005052082979133No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG27410.10965048258613523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82250.086.640051
ATAGCGG3800.079.3778151
CGTAGGG6750.078.167362
AGGGATG53450.077.4601445
ACGGGTA3900.074.892494
TAGGGCA16100.073.737064
GAGGGAT42700.072.374764
CGAAGGG24750.071.94952
AAGGGTA23900.071.551694
AGTAGGG41900.071.507942
ATAGGGC18550.071.3630753
AAGGGAT54850.071.287384
AGGGTAC12800.070.859395
TAACGGG7350.070.504452
TAGGGTA11600.070.258574
AGGGTAT20100.069.907735
TAGAGGG50450.069.4741062
GTAGGGA23400.069.255423
TAAGGGA26300.068.9633
TAGGGCG4800.068.7017754
Produced by FastQC (version 0.11.5)