FastQCFastQC Report
Mon 27 Feb 2023
SRR3127663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283375
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193830.8488750205288226No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC53220.23307603875841681No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG46900.20539771172058902No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG44020.19278480319702196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76650.085.839891
CGTAGGG4450.079.213542
AAGGGAT59250.076.146964
AGGGATT44250.076.044785
TAGGGCA17400.075.897374
ATAGCGG4600.075.6584551
AGGGATG42600.075.349565
CGAGGGA17700.075.1420063
ACGGGAT9300.074.285494
ATAGGGA33100.074.116033
TAAGGGA30300.073.985293
AAGGGCA47500.073.414184
CGAAGGG19400.073.1652
AAGGGAC29800.072.861044
GAGGGAT51600.072.499164
AGAGGGC40150.072.3390053
GTAGGGC15000.072.061983
AGGGCGT5100.071.877685
TAGGGCG5700.071.7321854
AGGGATC30200.071.11795
Produced by FastQC (version 0.11.5)