FastQCFastQC Report
Mon 27 Feb 2023
SRR3127662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715124
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264600.9745411259301602No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC53010.19523970175947766No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG44450.1637125965517597No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG43340.15962438547926355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103400.085.5471341
ATAGCGG4950.080.794511
AAGGGAT75200.076.494034
TAAGGGA35050.075.623213
AGGGATT55650.074.8225565
AGGGATG54500.074.418055
CGTAGGG6100.073.961452
ATAGGGA40550.073.478853
AGGGCGA17400.073.195445
AGGGCAT34050.073.01345
TAGGGCA22700.072.8753744
CGAGGGA21300.072.8112263
GAGGGAT59700.072.029564
AGGGATC37350.071.8471455
CGAAGGG23700.071.386842
GTAGGGA30500.071.341983
TAGAGGG50550.071.307992
ACGGGAT14650.070.89554
TAGGGAT33750.070.738184
AGAGGGA96800.070.30033
Produced by FastQC (version 0.11.5)