FastQCFastQC Report
Mon 27 Feb 2023
SRR3127660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615585
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458861.7543302932231224No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT33080.12647266290332757No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG26470.10120106974156834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143450.088.199731
TAGGGCA19350.077.2362444
ATAGGGC20100.077.160123
AGGGATG41000.071.184065
GAATAGG15200.070.242151
GTACGGG5450.068.987192
AGTAGGG42950.068.49932
TAGAGGG50500.068.216212
ATAGCGG5200.067.8380661
ATAGAGG19150.066.806031
GATAGGG40250.066.6722642
AAGAGGG95150.066.483092
CGTAGGG6950.066.269892
GGTAAGG15000.066.161711
TATAGGG22200.066.050572
ATAGGGA24850.066.004583
TAGACGG3850.065.9703141
AAGGGCG12150.065.757854
AGAGGGC42650.065.5651253
GGATAGG19950.065.541661
Produced by FastQC (version 0.11.5)