FastQCFastQC Report
Mon 27 Feb 2023
SRR3127659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155282
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT601651.906802624931781No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT32160.10192432879216501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT197600.087.70281
TAGGGCA21050.072.78474
ATAGGGC19900.071.795143
TAAGGGA31950.071.047843
AGGGATG51150.070.748895
CGTAGGG8150.069.199842
ATAGCGG6400.069.092151
TAGAGGG61500.068.242962
GAGGGAT44950.068.169874
AGTAGGG56000.067.811732
TAGGGTA9050.067.510194
AAGAGGG114650.066.244342
GAATAGG18800.066.058231
ATAGGGA28650.065.944113
ATAGAGG23200.065.89861
GTACGGG6750.065.449382
ACGGGTA4600.065.3877944
AGGGCAT32950.065.325785
TAGTAGG16150.065.246361
AGAGGGA77800.064.5762253
Produced by FastQC (version 0.11.5)