FastQCFastQC Report
Mon 27 Feb 2023
SRR3127658.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127658.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438999
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449471.8428461840287755No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT29530.1210742603830506No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG24410.1000820418540557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140350.087.91511
TAGGGCA15750.075.376984
ACGGGTA2850.074.3851854
ATAGGGC17300.073.525243
AGGGATG37150.073.116965
TAGACGG3100.071.456611
TAGAGGG46800.071.169242
TAGGGTA7550.071.133924
AGAGGGC40000.069.017053
AGATAGG14100.068.8579941
AGTAGGG40300.068.853982
ATAGCGG4450.068.842971
CGAAGGG24900.068.679342
TAAGGGA23750.068.037653
GAATAGG14800.067.830371
CGAGGGA13550.067.449883
ATAGGGA21950.066.7489853
AAGGGAT39050.066.7149054
CGTAGGG6850.066.711392
AGTAAGG17500.066.521971
Produced by FastQC (version 0.11.5)