FastQCFastQC Report
Mon 27 Feb 2023
SRR3127656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034797
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830354.080751052807725No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT55710.2737865251423115No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT51430.25275248587451227No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT41750.20518017276416273No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG29820.14655024555275045No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC24930.12251836423977427No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC20430.10040313603764896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT233100.090.549991
AGGGATG46250.076.8283845
TAGGGCA15900.074.788534
CGTAGGG6350.073.276112
GAGGGAT32400.072.533164
AGGGATC16350.072.442665
ATAGGGC15400.072.028253
GGATGGC36050.070.665217
AAGGGAT47000.070.102214
GTAGGGC9800.070.022623
AGAGGGC35050.069.8652653
TAAGGGA23150.069.4363253
ATAGGGA24600.068.9737243
AGTAAGG14750.068.8868561
TAGGGAT22150.068.539414
GTAGGGA19900.068.258443
AGTAGGG30950.067.8809362
AAGGGCA39050.067.763994
TAGAGGG36550.067.5107352
GTACGGG3900.067.4876562
Produced by FastQC (version 0.11.5)