FastQCFastQC Report
Mon 27 Feb 2023
SRR3127655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566243
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT621983.9711590091703526No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT51250.32721614717511904No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT46560.2972718792677765No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT39150.2499612129152373No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG27600.17621786657625926No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC22360.14276201074801292No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC19380.1237355889220255No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC18360.1172231895050768TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT171450.090.276881
AGGGATG34850.078.617635
ATAGCGG2700.078.528531
GAGGGAT27250.075.3536154
CGAGGGA10800.074.132613
GTAGGGA12750.072.767963
AGGGATC15250.072.418955
TAACGGG5100.072.029192
AAGGGAT34700.071.933394
ATAGGGA16700.071.913073
ATAGGGC10500.071.765353
GGATGGC26850.070.361647
TAGGGAT16250.070.1368264
AGAGGGC26350.070.063143
ATGCGGG6950.069.796972
TAGGGCA10950.069.676294
GAATAGG8250.069.6762161
AGTAGGG24050.068.734722
GTAGGGT5350.068.6633453
AAGGGCA30550.068.6013954