FastQCFastQC Report
Mon 27 Feb 2023
SRR3127654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106421
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188490.8948353629212773No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32460.15410024871571257No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG28710.13629753976057019TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG27750.13174004626805375TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC25260.11991904752183917TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65600.083.664951
CGTAGGG5700.075.857892
TAGGGCA15450.075.1356354
ATAGGGC14250.072.88783
GACCGAT1300.072.3025748
TAGAGGG38850.071.6173862
AGGGATG45750.071.6027155
CGAAGGG20050.071.025782
TAGCGGG9600.070.008792
AGAGGGC33450.069.54833
AAGAGGG78400.069.059622
AGGGCAT22300.068.917685
GGTAAGG12350.068.165181
AAGGGAT42600.068.069574
AGTAGGG37000.067.957882
TAGGGTA8100.067.88574
GTAGGGA16300.067.757553
TAGGGAT17350.067.449284
ATAGGGT11050.067.200473
AATAGGG35700.067.14132
Produced by FastQC (version 0.11.5)